課程名稱: 細胞微機電及微流體導論
(Introduction to Cellular BioMEMS and Biomicrofluidics)

Reference textbook: Introduction to BioMEMS, Albert Folch, CRC Press; 1st ed. (August 21, 2012)

Ch1 How do we make small things?
Ch2 Micropatterning of substrates and cells
Ch3 Microfluidics
Ch5 Cell-based chips for biotechnology
Ch6 BioMEMS for cell biology
Ch7 Tissue microengineering

Reference textbook：Essential Cell Biology, Bruce Albert, et al. Garland Science, 2nd ed. (September 25, 2003)

Ch17 Cytoskeleton
Ch19 Cell division

Introductory Biomechanics - From Cells to Organisms,
C. Ross Ethier and Craig A. Simmons, Cambridge University Press (April 9, 2007)

Ch2 Cellular biomechanics
Ch3 Hemodynamics
Ch4 The circulatory system
Ch5 Interstitial fluid flow

Fundamental of Microfabrication, Marc Madou, CRC Press

Ch1 Lithography
Ch2 Pattern transfer with dry etching technologies
Ch4 Wet bulk micromachining
Ch5 Surface micromachining.

Theoretical Microfluidics, HenrikBruus, Oxford University Press, (Nov 17, 2007)

Ch2 Governing equations
Ch3 Basic flow solutions
Ch4 Hydraulic resistance and compliance
Ch5 Diffusion

Course materials: Lecture notes and journal papers

1. Cellular biomechanics: anatomy and physiology of cells, mechanics of cytoskeleton, cell-matrix interaction, focal adhesions, mechanical model, mechanoreceptors, mechanical behavior of cells: mitosis, migration, and introduction to infection induced cell abnormality.
2. Tissue Engineering: microcirculation, capillary anatomy, diffusion and convection, Starling law, osmotic pressure, interstitial flow, basics of angiogenesis and vasculogenesis.
3. BioMEMS: Photolithography, bulk micromachining, surface micromachining, micro-molding, plastic manufacturing.
4. Microfluidics: scaling laws, surface to volume ratio, hydraulic resistance, wall shear stress, diffusion, capillary flow, hydrodynamics in porous media.
5. Special topic: Cell-based chip for biotechnology - bioreactors, studies of mechanics of abnormal cells, cell sorting, cell trapping.
6. Special topic: BioMEMS for cell biology - substrate dependency of cells, cell-cell contact, cell migration.
7. Special topic: Tissue microengineering - 3D culture, angiogenesis, vasculogenesis, organ on a chip.

1. Introduce how to use microfabrication methods to make masters, microfluidic devices, and micro-structures for cell mechanics, organ-on-a-chip, and microphysiological systems.
2. Discuss the necessary properties of the common materials used for studying cellular behaviors, cell-cell interactions, and developing organ tissue on a chip.
3. Discuss the methods used to study cell mechanics and the importance of their findings for the field of cell mechanics.
4. Explain how to apply cell mechanics to the design of BioMEMS and Biomicrofluidic devices.
5. Understand the applications of BioMEMS and Biomicrofluidics on organ-on-a-chip and microphysiological systems.